EFFECTS OF TYPE 2 DIABETES MELLITUS INDUCTION AND OF MODERATE PHYSICAL EXERCISE ON THE HEPATIC METABOLISM AND OXIDATIVE STRESS IN WISTAR RATS

Autores/as

  • Denise Silva de Oliveira Universidade Federal de Jataí, Instituto de Biociências, Jataí-GO
  • Júlia Matzenbacher dos Santos Detroit R&D, Inc, 2727, 2nd street, Detroit, MI, 48208 USA. Fairmont State University, Department of Education, Health and Human Performance, Fairmont, WV, USA. Fairmont State University- Department of Education, Health and Human Performance, 1201 Locust Avenue, Fairmont, WV 26554.
  • Kayorrany Gomes Galvão do Prado Instituto de Biociências, Universidade Federal de Jataí (UFJ), Jataí, GO, Brasil
  • Andreia Vitor Couto Amaral Pós-graduação em Biociência Animal, Universidade Federal de Jataí (UFJ), Jataí, GO, Brasil.
  • Sandra Aparecida Benite-Ribeiro Pós-graduação em Biociência Animal, Universidade Federal de Jataí (UFJ), Jataí, GO, Brasil. Pós-graduação de Ciências Aplicada à Saúde, Universidade Federal de Jataí (UFG), Jataí, GO, Brasil. CEP 75801-615 BR 364, km 195, nº 3800. sandrabenite@ufg.br

DOI:

https://doi.org/10.21527/2176-7114.2020.39.55-61

Palabras clave:

Insulin resistance; blood glucose; high fat diet; amino acid metabolism.

Resumen

AIMS: The aim of the study was to verify if the induction of Type 2 Diabetes Mellitus (T2DM) by the association of streptozotocin and high fat diet causes hepatic alterations and if physical exercise can improve these alterations.  METHODS: Forty-six Wistar rats were divided into two groups. One group received a diet containing 60% of fat for 9 weeks and on the 14th day they were injected with a single dose of streptozotocin (25mg/kg). The other group, named control group, received a standard diet and a single dose of citrate buffer was given. Animals were them divided into two subgroups: one sedentary (S) and the other submitted to physical exercise (PE), totalizing four groups: C-S; C-PE; HF-S; HF-PE. After euthanasia blood and liver were collected. Plasma and hepatic concentrations of triacylglycerol and very low density lipoprotein; enzyme alanine transaminase, aspartate transaminase and arginase; urea, lactate and 8-isoprostane (an oxidative stress marker) were dosed. RESULTS: Capillary glucose was significantly higher in HF-S and HF-PE group animals compared to C-S and C-PE groups. There were no significant differences in alanine transaminase, arginase, oxidative stress and lactate concentration among the groups. Plasma urea concentration and hepatic aspartate transaminase concentration was lower in HF-S and HF-PE than in C-PE. Concerning liver triacylglycerol and very low density lipoproteins, both were higher in the HF-PE group when compared to the C-S. CONCLUSIONS: We conclude that diet was decisive for the aforementioned parameters alterations and that physical exercise protocol was not efficient to improve analyzed parameters.

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Publicado

2020-10-22

Cómo citar

Oliveira, D. S. de, Santos, J. M. dos, Prado, K. G. G. do, Amaral, A. V. C., & Benite-Ribeiro, S. A. (2020). EFFECTS OF TYPE 2 DIABETES MELLITUS INDUCTION AND OF MODERATE PHYSICAL EXERCISE ON THE HEPATIC METABOLISM AND OXIDATIVE STRESS IN WISTAR RATS. Revista Contexto &Amp; Saúde, 20(39), 55–61. https://doi.org/10.21527/2176-7114.2020.39.55-61