Glycemia, temperature, and hematological variables as biomarkers in a mouse model of experimental sepsis
DOI:
https://doi.org/10.21527/2176-7114.2026.51.15465Keywords:
Sepsis, Clinical Markers, Hematology, Mouse ModelAbstract
Sepsis is a life-threatening condition characterized by infection and widespread inflammation, resulting in multiple organ dysfunction syndrome (MODS). This study aimed to evaluate whether temperature, body weight, glycemia, hematological parameters, and their ratios can be used as biomarkers in a mouse model of experimental sepsis. An experimental animal study using mice was conducted, divided into two groups: Control group (CTRL) and Sepsis group (SEP). Animals in the control group received 0.9% saline solution, whereas those in the sepsis group received an intraperitoneal injection of 20% fecal solution (1 mg/g). Clinical and laboratory biomarkers were assessed at 0, 4, 12, and 24 hours throughout the experimental protocol. It was demonstrated that, as early as 4 hours after sepsis induction, hypothermia and decreased blood glucose levels were observed in the sepsis group compared to the control group. At 24 hours, the sepsis group showed reductions in mean corpuscular volume (MCV) and platelet counts. In addition, an increase in the neutrophil-to-lymphocyte and platelet ratio (NLPR) was identified. Thus, these findings demonstrate that hypothermia, glycemia, and hematological parameters may serve as important biomarkers for the diagnosis and monitoring of sepsis.
References
1. Seymour CW, Liu VX, Iwashyna TJ, Brunkhorst FM, Rea TD, Scherag A, et al. Assessment of Clinical Criteria for Sepsis: For the Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA [Internet]. 23 de fevereiro de 2016;315(8):762–74. Disponível em: https://doi.org/10.1001/jama.2016.0288
2. World Health Organization. Sepsis [Internet]. 2021 [citado 27 de julho de 2023]. Disponível em: https://www.who.int/news-room/fact-sheets/detail/sepsis
3. Singer M, Deutschman CS, Seymour CW, Shankar-Hari M, Annane D, Bauer M, et al. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA [Internet]. 23 de fevereiro de 2016;315(8):801–10. Disponível em: https://doi.org/10.1001/jama.2016.0287
4. ILAS. DIA MUNDIAL DA SEPSE/SOBRE A SEPSE NO MUNDO – ILAS [Internet]. 2025. Disponível em: https://ilas.org.br/dia-mundial-da-sepse/
5. He RR, Yue GL, Dong ML, Wang JQ, Cheng C. Sepsis Biomarkers: Advancements and Clinical Applications—A Narrative Review. Vol. 25, International Journal of Molecular Sciences. Multidisciplinary Digital Publishing Institute (MDPI); 2024.
6. Zhong Y, Zhong L, Zhou Y, Liao Y, Deng J. Dynamic neutrophil-to-lymphocyte-platelet ratio trajectories predict 30-day and 1-year mortality in sepsis: a retrospective cohort study based on MIMIC-IV 2.2. BMC Infect Dis [Internet]. 2025;25(1). Available from: https://doi.org/10.1186/s12879-025-10987-3
7. Ministério da Ciência T e IN de C de EA. https://ww3.icb.usp.br/pes/wp-content/uploads/2024/11/DBCA-2024.pdf. 2024. Diretriz Brasileira para o Cuidado e a Utilização de Animais em Atividades de Ensino ou de Pesquisa Científica - DBCA.
8. Machado Sulzbacher M, Bender dos Santos A, Basso R, Hirsh G, Ludwig M, Heck T. Efeitos do tratamento com glutamina via enteral em modelo animal de sepse. Saúde (Santa Maria). 15 de agosto de 2018;44.
9. Bouglé A, Rocheteau P, Hivelin M, Haroche A, Briand D, Tremolada C, et al. Micro-fragmented fat injection reduces sepsis-induced acute inflammatory response in a mouse model. Br J Anaesth [Internet]. 2018;121(6):1249–59. Disponível em: https://www.sciencedirect.com/science/article/pii/S0007091218303192
10. Shrum B, Anantha R V, Xu SX, Donnelly M, Haeryfar SMM, McCormick JK, et al. A robust scoring system to evaluate sepsis severity in an animal model. BMC Res Notes [Internet]. 2014;7(1):233. Disponível em: https://doi.org/10.1186/1756-0500-7-233
11. Sulzbacher MM, Ludwig MS, Heck TG. Estresse oxidativo e diminuição de HSP70 tecidual envolvidos na gênese da sepse: HSP70 como alvo terapêutico. Rev Bras Ter Intensiva. 2020;32.
12. HORIBA Diagnostics. Manual do usuário: ABX Micros 60.
13. Zhao Y, Zhang B. Association between body temperature and all-cause mortality in patients with sepsis: analysis of the MIMIC-IV database. Eur J Med Res. dezembro de 2024;29:630.
14. Barichello T, Giridharan V V., Catalão CHR, Ritter C, Dal-Pizzol F. Neurochemical effects of sepsis on the brain. Vol. 137, Clinical Science. Portland Press Ltd; 2023. p. 401–14.
15. Santos-Junior NN, Costa LHA, Catalão CHR, Kanashiro A, Sharshar T, Rocha MJA. Impairment of osmotic challenge-induced neurohypophyseal hormones secretion in sepsis survivor rats. Pituitary [Internet]. 2017;20(5):515–21. Disponível em: https://doi.org/10.1007/s11102-017-0812-z
16. Shimazui T, Nakada TA, Walley KR, Oshima T, Abe T, Ogura H, et al. Significance of body temperature in elderly patients with sepsis. Crit Care. junho de 2020;24.
17. Kushimoto S, Gando S, Saitoh D, Mayumi T, Ogura H, Fujishima S, et al. The impact of body temperature abnormalities on the disease severity and outcome in patients with severe sepsis: an analysis from a multicenter, prospective survey of severe sepsis. Crit Care [Internet]. 2013;17(6):R271. Disponível em: https://doi.org/10.1186/cc13106
18. Wang J, Zhu CK, Yu JQ, Tan R, Yang PL. Hypoglycemia and mortality in sepsis patients: A systematic review and meta-analysis. Heart & Lung: The Journal of Cardiopulmonary and Acute Care [Internet]. 1o de novembro de 2021;50(6):933–40. Disponível em: https://doi.org/10.1016/j.hrtlng.2021.07.017
19. Kushimoto S, Abe T, Ogura H, Shiraishi A, Saitoh D, Fujishima S, et al. Impact of blood glucose abnormalities on outcomes and disease severity in patients with severe sepsis: An analysis from a multicenter, prospective survey of severe sepsis. PLoS One [Internet]. 11 de março de 2020;15(3):e0229919-. Disponível em: https://doi.org/10.1371/journal.pone.0229919
20. Shao Y, Shao F, Zhou J, Fang S, Zhu J, Li F. The association between hypoglycemia and mortality in sepsis and septic shock: A systematic review and meta-analysis. Adv Clin Exp Med. 30 de junho de 2023;33.
21. Sulzbacher MM, Sulzbacher LM, Passos FR, Bilibio BLE, de Oliveira K, Althaus WF, et al. Adapted Murine Sepsis Score: Improving the Research in Experimental Sepsis Mouse Model. Maciejczyk M, organizador. Biomed Res Int [Internet]. 2022;2022:5700853. Disponível em: https://doi.org/10.1155/2022/5700853
22. Park S, Kim DG, Suh GY, Kang JG, Ju YS, Lee YJ, et al. Mild hypoglycemia is independently associated with increased risk of mortality in patients with sepsis: a 3-year retrospective observational study. Crit Care [Internet]. 2012;16(5):R189. Disponível em: https://doi.org/10.1186/cc11674
23. Piagnerelli M, Cotton F, Nuffelen M Van, Vincent JL, Gulbis B. Modifications in Erythrocyte Membrane Protein Content Are Not Responsible for the Alterations in Rheology Seen in Sepsis. Shock [Internet]. 2012;37(1). Disponível em: https://journals.lww.com/shockjournal/fulltext/2012/01000/modifications_in_erythrocyte_membrane_protein.3.aspx
24. Zhang J, Xie JF, Chen H, Li C, Mo M, Qiu H, et al. Development and validation of a clinical score combining the sequential organ failure assessment score with inflammation-based markers to predict outcome of patients with sepsis. Am J Transl Res. 15 de março de 2023;15:1789–97.
25. Huang Z, Fu Z, Huang W, Huang K. Prognostic value of neutrophil-to-lymphocyte ratio in sepsis: A meta-analysis. Am J Emerg Med [Internet]. 2020;38(3):641–7. Disponível em: https://www.sciencedirect.com/science/article/pii/S0735675719306710
26. Agnello L, Giglio R, Bivona G, Scazzone C, Gambino C, Iacona A, et al. The Value of a Complete Blood Count (CBC) for Sepsis Diagnosis and Prognosis. Diagnostics. 12 de outubro de 2021;11:1881.
27. Shi Y, Yang C, Chen L, Cheng M, Xie W. Predictive value of neutrophil-to-lymphocyte and platelet ratio in in-hospital mortality in septic patients. Heliyon. 2022;8(11).
28. Si Y, You S, Lei Q, Liu C, Zhang J. Time series analysis between platelet counts and 60-day mortality in sepsis patients with thrombocytopenia: a retrospective cohort study. BMC Infect Dis. dezembro de 2025;25.
29. Schupp T, Weidner K, Rusnak J, Jawhar S, Forner J, Dulatahu F, et al. Diagnostic and prognostic role of platelets in patients with sepsis and septic shock. Platelets [Internet]. 31 de dezembro de 2023;34(1):2131753. Disponível em: https://doi.org/10.1080/09537104.2022.2131753
30. Andrade AMM de, Vieira MS, Horta RD, Chaves MA. Coagulação intravascular disseminada: uma abordagem diagnóstica. Rev Med (Rio J) [Internet]. 4 de outubro de 2021;100(4):366–74. Disponível em: https://www.revistas.usp.br/revistadc/article/view/185363
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Copyright (c) 2026 Samara Cristine Knebel, Lucas Machado Sulzbacher, Maicon Machado Sulzbacher, Pauline Brendler Goettems Fiorin, Mirna Stela Ludwig, Vítor Antunes de Oliveira, Thiago Gomes Heck, Matias Nunes Frizzo
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